supm2 cell line  (DSMZ)

Journal: Cell Reports Medicine

Article Title: A patient-derived T cell lymphoma biorepository uncovers pathogenetic mechanisms and host-related therapeutic vulnerabilities

doi: 10.1016/j.xcrm.2025.102029

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Article Snippet: SUPM2 cell line , DSMZ , ACC 509.

Techniques: Transduction, Recombinant, Saline, Software, Sterility, Transferring, Ointment

Journal: Nature medicine

Article Title: Wiskott-Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma.

doi: 10.1038/s41591-018-0262-9

Figure Lengend Snippet: Fig. 1 | WASP and WIP are selectively down-regulated in ALCL. a, Gene-expression profiling analysis of ALK, WASP, WIP, N-WASP, CDC42 and TNFRS8 (CD30) on different cases of human T cell lymphomas: AITL, n = 40; PTCL-NOS, n = 74; ALK– ALCL, n = 24, ALK+ ALCL, n = 30. The boxes represent the first and third quartiles and the line represents the median. The whiskers represent the upper and lower limits of the range (ALK+ ALCL versus AITL, ****P = 5.85 × 10−6; ALK+ ALCL versus PTCL-NOS, ****P = 6.08 × 10−12; ALK+ ALCL versus ALK– ALCL, **P = 0.0075; significance was determined by unpaired, two-tailed Student’s t-test). TNFRS8 (CD30) is strongly expressed in ALK– and ALK+ ALCL but not in other TCL subtypes. b, Representative H&E staining and immunohistochemistry stainings performed with the indicated antibody on human T cell lymphoma subtypes. The number of human T cell lymphoma samples analyzed is reported in Fig. 1c. WASP antibody was validated in formalin-fixed samples on samples with inducible WASP expression (Supplementary Fig. 7f). WIP antibody cross reacts with mouse WIP and was validated on WIP knockout cells (Supplementary Fig. 1b). Scale bar, 100 μm. Insets: high-magnification images. c, WASP and WIP expression in human T cell lymphoma subtypes. AITL, n = 20; PTCL-NOS, n = 20; ALK– ALCL, n = 29; ALK+ ALCL, n = 43 for WASP and n = 31 for WIP; other TCL are natural killer (NK)/T cell lymphoma, nasal-type, n = 3 and hepatosplenic γδ T cell lymphoma, n = 3. The number of patient samples is indicated for each lymphoma subtype. WASP and WIP expressions were quantified by immunostaining. Normal expression, expression equal to surrounding reactive T cells; low expression, decreased expression compared to surrounding reactive T cells; and no expression, absence of expression in lymphoma cells.

Article Snippet: Human ALK+ ALCL cell lines (TS, SU-DHL1, JB6, Karpas-299, DEL, SUP-M2 and L82) and ALK− cell lines (MAC-1, FePD and Jurkat) ALCL cell lines were obtained from DSMZ (German collection of Microorganisms and Cell Cultures).

Techniques: Gene Expression, Two Tailed Test, Staining, Immunohistochemistry, Expressing, Knock-Out, Immunostaining